All Maharal Geanealogy is detailed here

http://www.hebrewbooks.org/pdfpager.aspx?req=33676&st=&pgnum=1&hilite

(Hebrew)

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Betzalel ben Chaïm LOEW (LOEB) was born about 1480. Unlike his young brothers, stayed behind to help his father and did not pursue studies in Poland. Betzalel married the daughter of Rabbi Chaim ISSEMHEIMER or married daughter of Rabbi Yitzchak KLOBER of Worms. They had four sons and three daughters, The Maharal of Prague was the youngest son:

• daughter :oew m ?
• Rabbi Chaim ben Betzalel LOEW (b. 1506? 1510? Worms, d. 1588, Rabbi of Friedberg).
• Rabbi Sinai LOEW (b. ca 1508 Worms, d. 1607 Kolin).
• Rabbi Shimshon (Samson) ben Betzalel LOEW (b. 1522? 1510? Prague, d. Kremitz),
• daughter married Rabbi Zecharia Mendle KLOIZNER the Elder.
• daughter married Rabbi Avigdor KARA.
• Maharal of Prague, Rabbi Yeudah (Judah) LOEW ben Betzalel (b. 1525 Posen or Pesach Eve. 1512 Worms, d. 22 August 1609 Prague, or 17 September 1609 according the Jahrzeit date of 18 Elul 5369. [The Maharel] [Der Hohe Rabbi LOWE] [High Rabbi Lowe] married Perla SHMELKES (b. 1528 Prague, d. 5 May 1610 Prague) daughter of Samuel SHMELKES Reich son of Jakob SCHMELKES.
• Sefer Imrei Ash suggests, most probably by mistake, another undocumented son by the name of Reb Yosef LOEW, Rabbi of Naishtat

-------------------- הערה לגבי בנו שמשון: "רבי שמשון מאוסטרופול היה סבו של רבי ליבר הגדול מברדיטשב ונכדו של רבי שמשון בן בצלאל ליווה, אחיו של המהר"ל מפראג" ראה עוד בפרופיל של שמשון הבן.