Comfort (Williams) Hester is unlikley to have had a sister also named Comfort. She did, however, have a daughter named Comfort Janes Hester who married a Singletary. John Hester's will mentions his wife's name as Comfort and lists his daughters:
".... To my daughters Comfort, Sarah, Mirium, Mary, Hannah" in his will. Notice...only one daughter named Comfort and that would be the daughter who married Thomas Singletary.
You can read John's entire will in his profile Overview and also attached to his wife Comfort and their daughter Comfort Jane.