Siblings: Ann Middleton, Katherine Harwood (born Middleton), Andrew Middleton, Henry Middleton, Hester Browning (born Middleton), Mary Birkhead (born Middleton), Edward Middleton, Martha Middleton, William Middleton, Thomas Middleton, Ellen Dykes (born Middleton)
Wife: Mary Middleton
Children: Edward Middleton, Thomas Middleton, Mary Middleton