Jane (Rudd) Waln

The English ancestry of Nicholas Wain, early Quaker immigrant to Pennsylvania, with an account of the Rudd and Turner families by Stewart Baldwin January, 1993

https://dcms.lds.org/delivery/DeliveryManagerServlet?dps_pid=IE1073612

That Jane (Rudd) Wain was the mother of Anne and Edward is clear, since their birth records say so. However, I know of no source which actually proves that Jane was the mother of Nicholas. Since no other marriage of Richard Wain is known, or even vaguely indicated, the chances of an error here are very slim. Even though this is a very picky point, it at least needs to be mentioned.

• Reference: WikiTree Genealogy - SmartCopy: Jun 30 2016, 1:48:20 UTC https://archive.org/details/colonialrevoluti00jord - this book clearly gives Richard and Jane as Nicholas's parents. (See pages 200-225 of Volume 1 - the whole Waln family is discussed)