Parents: Benjamin Webb Anstie, Hannah Anstie (born Overbury)
Siblings: Mary Anstie, Sarah Anstie, John Anstie, Benjamin Anstie, Peter Sharp Anstie, Hannah Smith (born Anstie), Ann Anstie, Paul Anstie, Henry Anstie, George Washington Anstie, James Overbury Anstie
Husband: Thomas Overbury
Children: Sarah Overbury, Hannah Overbury, Anne Overbury, Josiah Overbury, Mary Overbury, Thomas Overbury, John Overbury, Amelia Overbury