knez Batić Mirković, knez Mirko Radojević

Abstract number: P17

PREDICTION OF Y HAPLOGROUP IN ANALYSIS OF HUMAN SKELETAL REMAINS FROM ARCHAEOLOGICAL SITES IN BOSNIA AND HERZEGOVINA

Abdurahim Kalajdžić, Mirela Džehverović, Edin Bujak, Amela Pilav, Jasmina Čakar
University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Sarajevo,
Bosnia and Herzegovina
University of Sarajevo, Faculty of Philosophy, Department of Archeology, Sarajevo, Bosnia and Herzegovina

Archaeological skeletal remains represent a valuable source of information in the context of determining haplogroups. Y STR ancient DNA (aDNA) analysis is often used for identification, disputable male kinship testing and monitoring migration patterns. The most common Y haplogroup among recent males in Bosnia and Herzegovina is I haplogroup (50%) with I-P3 subhaplogroup. Main goals of this study were successful isolation of aDNA from skeletal remains found at two archaeological sites (Divičani and Kopošići) and prediction of Y haplogroups for obtained Y STR haplotypes. DNA was isolated from teeth and bones samples using an optimized phenol/chloroform DNA extraction procedure, preceding decalcification with EDTA. Quantification of samples was performed with a Quantifiler Duo quantification kit for determining the exact concentration of DNA and potential presence of the inhibitors. PowerPlex® Y23 System was used for amplification of 23 Y STR loci and analysis was performed on ABI PRISM 310 Genetic Analyzer. Analysis of the generated haplotypes and prediction of the haplogroups was done using YHRD database, Y-DNA Haplogroup Predictor – NEVGEN and Athey: Haplogroup predictor – HAPEST. Partial DNA profiles were obtained for all samples. Comparison of given Y DNA profiles concluded that four persons were in a kinship relations by a paternal line. Subsequent analysis showed that all samples from Kopošići and Divičani sites belong to haplogroup I2a and subhaplogroup I2a1b3 except one case from Kopošići which belongs to haplogroup R1a. Prediction results from both online software’s used in this study were in concordance, however predictor NEVGEN calculates subgroups of haplogroup I2a. These preliminary results indicate that genetic structure of male population in medieval B&H is similar to the recent one. Our results also showed that both of these software’s can be used for predicting haplogroups, however HAPEST is the recommended one.

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