Elizabeth I of England is James VI King of Scots, James I King of England & Ireland's first cousin twice removed!

James VI King of Scots, James I King of England & Ireland  → Henry Stewart, Lord Darnley (his father) → Lady Margaret Douglas, Countess of Lennox (his mother) → Margaret Tudor, Queen consort of Scots (her mother) → Henry VIII, King of England (her brother) → Elizabeth I of England (his daughter)

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