The Leader of the Opposition (French: Chef de l'Opposition) in Manitoba is the MLA in the Legislative Assembly of Manitoba who leads the political party recognized as the Official Opposition. This status generally goes to the leader of the second largest party in the Legislative Assembly.

Leaders of the Opposition

Conservative Leaders

• William Alexander Macdonald (1892-1893)
• John Andrew Davidson (1893-1894)

Independent Leader

• James Fisher (1894-1896)

Conservative Leader

• Rodmond Palen Robin (1896-1900)

Liberal Leaders

• Thomas Greenway (1900-1904)
• Charles Mickle (1904-1906)
• Charles Mickle (1908-1909)
• Tobias C. Norris (1910-1915)

Conservative Leaders

• Albert Prefontaine (1916-1920)
  • unknown as Conservative Leader Robert G. Willis did not have a seat in the Legislative Assembly (1920-1922)

Liberal Leader

• Tobias C. Norris (1922-1927)

Conservative Leaders

• Fawcett G. Taylor (1927-1933)
• William Sanford Evans (1933-1936)
• Errick Willis (1936-1940)

Independent Leader

• Lewis St. George Stubbs (1940-1941) (Not clear if he was given the title as he was the only member of the Legislature that was not in the government.

Anti-Coalition Conservative Leader

• Huntly Ketchen (1941-1943)

Manitoba Co-operative Commonwealth Leaders

• Seymour J. Farmer (1943-1947)
• Edwin Hansford (1948-1950)

Progressive Conservative Leaders

• Errick Willis (1950-1954)
• Dufferin Roblin (1954-1958)

Liberal Leaders

• Douglas Lloyd Campbell (1958-1961)
• Gildas Molgat (1961-1969)

Progressive Conservative Leaders

• Walter Weir (1969-1971)
• Sidney Spivak (1971-1975)
• Donald Craik (1975-1976)
• Sterling Lyon (1976-1977)

New Democratic Leaders

• Edward Schreyer (1977-1979)
• Howard Pawley (1979-1981)

Progressive Conservative Leaders

• Sterling Lyon (1981-1983)
• Gary Filmon (1983-1988)

Liberal Leader

• Sharon Carstairs (1988-1990)

New Democratic Leader

• Gary Doer (1990-1999)

Progressive Conservative Leaders

• Gary Filmon (1999-2000)
• Bonnie Mitchelson (2000-2000)
• Stuart Murray (2000-2006)
• Hugh McFadyen (2006-2012)
• Brian Pallister (2012-2 May 2016)

New Democratic Leader

• Still to be chosen by Party